Elisa development dissertation
Skip to main content. Full Description View Document Title: Skeletal muscle damage is a elisa development dissertation occurrence and can lead to severe complications.
Early diagnosis of skeletal muscle damage is a leading please click for source in successful treatment and recovery. The ability more info rapidly, accurately, and non-invasively diagnose muscle damage is predicated on the ability to detect various biomarkers in blood.
Creatine kinase CK is a biomarker commonly used for this purpose, but limitations exist such as tissue specificity. Troponin I TnIgo here elisa development dissertation protein in elisa development dissertation, is a potential alternative.
Enzyme-linked immunosorbent assay ELISAa technique used to determine elisa development dissertation presence of a specific protein based on an antibody Ab -antigen Ag interaction, has elisa development dissertation successfully applied in the detection of cardiac TnI cTnI to diagnose myocardial infarction AMI. Attempts by other investigators to elisa development dissertation an ELISA assay for detecting skeletal muscle damage using skeletal troponin I sTnI have been unsuccessful because of cross-reactivity of the antibodies used with cTnI.
Elisa development dissertation goal of this elisa development dissertation was to initiate development of an ELISA for sTnI by testing four mammalian elisa development dissertation antibodies MAbs - 2G3, 8A12, 1F9, and 8F10 previously used for food science purposes and not for elisa development dissertation skeletal muscle damage.
These four MAbs were first screened elisa development dissertation the ability to elisa development dissertation human sTnI by testing them against a protein extract from human skeletal muscle; three of the MAbs 2G3, 8A12, 1F9 reacted positively.
Secondly, these three MAbs were then tested for cross-reactivity to cTnI with a beef heart protein extract, and only one of the three MAbs 1F9 was eliminated.
Finally, the MAbs 2G3 and 8A12 were tested to see if sTnI could be detected in serum samples from dissertation subjects with evidence of skeletal muscle damage based on CK values, but the results were inconclusive. In summary, the MAbs 2G3 elisa development dissertation link are specific for human sTnI, but experimental conditions need elisa development be further optimized to determine whether these two MAbs can detect sTnI in dissertation serum and therefore be used to develop an ELISA-based elisa development dissertation for diagnosing skeletal muscle damage.
A Thesis submitted to the Department of Elisa development dissertation, Food, and Exercise Sciences in partial fulfillment of the requirements for the degree dissertation Master elisa development dissertation Science.
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